RCM scanning unit is connected via standard C-mount adapters between the microscope and camera. The laser light is fed into the RCM unit via a single mode fibre connector (both standard connectors).
RCM can be mounted to any inverted microscope with a side-port. Motorised focussing unit (internal or external) is also needed in most applications. Currently, RCM has been tested with Nikon Ti & Ti2, Olympus IX and MiCube.
Please note that:
- RCM only mounts correctly to the original microscope manufacturers C-mount
- 50/50, 70/30 and 80/20 beamsplitters do not work with RCM, as they are not laser safe.
Contact us at firstname.lastname@example.org if you have any question regarding this.
In order to obtain the 170nm resolution, the highest NA (>1.40) lenses available have to be used. See the table below for the optimal combination of objective lens and camera resolution.
Theoretically, any microscope camera with a C-mount connector, external trigger, and similar specifications as the two above (the pixel size of 6.45 um is ideal for 60x & 100x imaging on the RCM), may work. Tested camera models include:
- Hamamatsu Flash 4 V2 (LT) and V3,
- Andor Zyla, 888, 897 & Ikon;
- Great Eyes GE 1024 1024 BI MID,
- PCO Edge 4.2,
- Photometrics Prime 95B.
Guide to combine camera and objective for best resolution
The table below lists the objectives and camera types we recommend to use with RCM. Using the objectives that are not listed here means that you will not achieve the improved lateral resolution through the rescanning principle. Also axial resolution and sectioning power will be reduced, due to a mismatch of the RCM pinhole size.
RCM drivers are available LabView (free of charge), uManager / ImageJ (Fiji) (free of charge), and Nikon NIS Elements (at cost).
Laser combiners: Omicron Lighthub, Toptica CLE, Coherent Calaxy and Nikon Lu-N4 The RCM laser coupling is compatible with single mode fibers with FC/APC connector and NA 0.11. Using other fiber connectors will degrade image quality.
Wavelengths: from 405 upwards. RCM is designed as a 4 colour system. We offer a flexible laser solution that can be equipped with 1 to 4 lasers. Laser lines can be upgraded at later stage as well. As the RCM is so sensitive, we only need low power lasers (max 25mW). The advantage of the low power lasers is the significantly longer lifetime.