Q: Why is scan speed 1 FPS?
A: Because we are using two sets of two galvano scanners. The perfect synchronisation takes time.
Q: Are there any limitations regarding the manufacturers of the inverted microscope that the RCM can be installed on?
A: No, all microscopes (Leica, Zeiss, Nikon and Olympus) can be used, but please check the physical space around the C-mount to see if the RCM unit will fit.
Q: Can I make the the acquisition in sequential and simultaneous mode?
A: At this moment we can acquire up to 4 images sequentially. We are working on the solution for acquiring 2 colours simultaneously (RCM-FRET tba).
Q: Why is it not possible to control the pinhole size?
A: In traditional confocal microscope, pinhole size is reduced to increase the lateral resolution. In the RCM, the lateral resolution is achieved by the re-scanning invention. So the pinhole size does not affect anymore the resolution – hence, the pinhole is now only used for optical sectioning. In the RCM the standard pinhole is 1.5* AU (but any size is available). To our experience, this size is most optimal due to excellent sectioning properties and the higher amount of light passing through.
*for 100x objective @ 510 nm
Q: What colours can be used with RCM?
A: The RCM is equipped with Quad-band dichroic mirror (45°) ZT405/488/561/640rpc and Quad-band emission filter ZET405/488/561/640m. On special request, RCM can be equipped with any other filter combination (single, dual triple or quad).
Q: Do I need a emission filter wheel in front of the camera?
A: The RCM unit does not have an internal emission filter-wheel. For most applications, channel separation on the excitation side (switching lasers on and off) is sufficient. An additional external filter wheel can be installed to further improve channel separation.