Why we bought our RCM
The RCM system configured for Markus Sauer’s Biomedical Imaging Facility at the University of Würzburg’s Biocenter is a typical offering from Confocal.nl. Based on an existing light microscope and accessories from Nikon, Zyla, Skyra and NIS software, the RCM module was the missing part to complete a super-resolution 3D scanning focal microscope for minimal investment.
The main user is Andreas Kurz and he describes his setup. “This is a fast, resolution-enhanced and live-cell capable system configured dSTORM for super-resolution performance. Our main reason for purchase was the promised performance of the complete package integrating our existing components at a very fair price. We have found the multi-colour capability with incredibly low laser power is very useful for live cell imaging.”
Time lapse with RCM: HeLa cells expressing EB1 tagged with YFP. 2 seconds time interval, (original time lapse 40 minutes), acquired with 8µW laser power (by Andreas Kurz).