Paramecium autofluorescence captured at 640nm excitation. Image is a stitched z-stack of 4×4 tiles. Captured using an 100x oil objective and rendered using the NIS-Elements 3D renderer.
Hatching mouse blastocyst. Indirect immunofluorescent staining: Alexa Fluor 488 – transcription factor GATA4 (primitive endoderm marker, green), Alexa Fluor 594 – transcription factor CDX2 (trophectoderm marker, red); nuclear staining: DRAQ5 (blue). Sample courtesy of Katarzyna Szczepańska (Department of Embryology, University of Warsaw).
Culex Pipiens (House mosquito). Autofluorescence captured at 640nm excitation. Imaged with the Re-scan Confocal Microsocope (RCM) with a Nikon 100x Plan Apo NA 1.45 objective. Stitched 25×25 region with 144 z-stacks per tile. Captured and stitched using NIS-Elements acquisition software. 3D rendering shows Raw RCM data.
Hela cell in mitosis stained for DAPI (blue), Actin (green) and Tubulin (red). Sample courtesy of Nicolas Touret, Faculty of Biochemistry, University of Alberta
This 3D image shows the sphere structure found in the Argolight slide. It shows that RCM has great resolution, also in Z. Sample courtesy: Axiom Optics (USA)