We improve your
Super-resolution. Low laser power. The solution for live cell imaging.
The fact that Confocal.nl allows for super-resolution in combination with very low laser intensity will be the winning strategy in live cell imaging.
Our solutions are as clear as our imaging
Sharper. Larger field of view. Easy to use.
Sharp images, a large field of view and easy to use? It’s possible with Confocal.nl. Get more from your samples, with a higher resolution and a higher sensitivity than conventional confocal microscopes and study live samples longer. Our Re-scanning Confocal Microscope (RCM) uses multiple laser pointers and a camera as a detector. The low laser power required is live cell friendly: it prevents phototoxicity in your live samples and photobleaching of your fluorophores. Creating long-term time lapses at a super-resolution was never this accessible.
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How we improve your imaging experience
Camera-Based Confocal Technology
Re-scan Confocal Microscopy (RCM) is a new super-resolution technique, based on standard confocal microscopy. It’s extended with an optical re-scanning unit that projects the image directly on a CCD- or sCMOS camera. By using a sensitive camera as detector, the signal-to-noise ratio of the RCM is 4 times higher than in standard PMT-based confocal microscopes. This new technology improves lateral resolution and strongly increases sensitivity, while maintaining the sectioning capability of a standard confocal microscope. Closing down the pinhole is no longer necessary to increase resolution thanks to the re-scan step.
It is excellent for biological applications where a combination of super-resolution and high-sensitivity is required.
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Discover our solutions
Clever design for brighter images and even lower laser power
RCM1 is a highly sensitive super-resolution confocal system. It’s capable of reaching a 120nm resolution after deconvolution (170nm raw). Study living cells in high detail with minimal phototoxicity and photobleaching.
RCM2 is, like the RCM1, a highly sensitive super-resolution confocal system. It uses digital scanning technology and bigger optics, which allows for brighter images and even lower laser power. Capture more biology with the same resolution.
RCM2.5 is our upgraded version of the RCM2, with a 5th additional laser. This version utilizes up to 5 lasers, extending over the Visible and Near-Infrared (NIR). Moreover, RCM2.5 enables you to use the latest advances in NIR dye development and look much deeper into your specimen. Experience complete experimental freedom and flexibility with our RCM2.5.
We kept cells alive for 61 hours under a microscope
The community challenged us
We took the challenge of long term live cell imaging and this is the result: 61 hours of live cell imaging without phototoxicity. HO1N1 cells expressing M itochondria-RFP through the Bacmam expression system. One image was taken every 10 seconds for 61 hours (giving a total of almost 22.000 images!). Laser power was measured to be 1 microwatt at the sample plane.
Movie taken by Jeroen Kole (Confocal.nl), sample courtesy Dandan Ma (ACTA, Amsterdam), equipment provided by Marko Popovic (Nikon Center of Excellence, Amsterdam University Medical centers, VUmc, Amsterdam.)
Become a partner
Start working on the future of microscopy
As a partner of Confocal, you’ll be working on the future of microscopy alongside a young and dynamic team. We’ll work together to create the best imaging solutions and to distribute them. Let’s make RCM accessible across the world.
A team of enthusiastic microscopists
We improve your imaging experience
Founded in 2016, Confocal.nl strives to improve the microscopic imaging experience of all researchers. With our international and open-minded team, we develop innovative and ingenious designs in cooperation with our users, we provide the most user-friendly upgrade technology for microscopes. This brings opportunities to researchers to deliver breakthrough science. With the passion we have for science we create value for all our stakeholders.
See for yourself how we improve your experience
In about 45 minutes to an hour, our experts
show you how our products perform live.