RCM scanning unit is connected via standard C-mount adapters between the microscope and camera. The laser light is fed into the RCM unit via a single mode fibre connector (both standard connectors). The RCM comes in two versions: RCM-Visible and RCM-NIR. Visible is designed to operate as a 4-colour system in the visible range (400-700nm). NIR is designed to operate in the Near-Infrared (785nm excitation). The RCM comes with Bypass mode to allow for fast focusing in epi-fluorescence.
RCM can be mounted to any inverted microscope with a side-port. Motorised focussing unit (internal or external) is also needed in most applications. Currently, RCM has been tested with Nikon Ti & Ti2, Olympus IX, MiCube, Zeiss Axio Observer and Leica DMi6000 & DMi8.
Please note that:
- RCM only mounts correctly to the original microscope manufacturers C-mount
- A 100/0 beam splitter is mandatory. 50/50, 70/30 and 80/20 beam splitters do not work with RCM, as they are not laser safe.
In order to obtain the 170 nm resolution, the highest NA (>1.40) lenses available have to be used. In addition to that the camera needs to have a suitable pixel size (see below).
Any microscope camera with a C-mount connector, external trigger, and similar specifications as the two first ones in the list below should work with the RCM. Please contact us, if you have a camera that you would like to test for the RCM compatibility.
Camera models tested to work with RCM:
- Hamamatsu Flash 4 V2 (LT) and V3,
- Andor Zyla, 888 and 897
- PCO Edge 4.2
- FLIR Chameleon 3 (Micromanager only, with custom driver)
A physical pixel size of 6.5 micron or lower is ideal for 60-100x objective lenses, as this results in sufficiently small sampling. At 100x objective magnification and 6.5 micron pixel the sampling is 43 nm at the sample plane.
RCM drivers are available for LabView (free of charge), Micro-Manager / ImageJ (Fiji) (free of charge), and Nikon NIS Elements (at cost).
Laser combiners: Omicron Lighthub, Toptica CLE, Coherent Galaxy, Nikon Lu-N4, Oxxius and Vortran. The RCM laser coupling is compatible with single mode fibers with FC/APC connector and NA 0.11. Using other fiber connectors will degrade image quality.
Wavelengths: For RCM-Visible lasers with a wavelength from 405 upwards are suitable. RCM is designed as a 4 colour system. We offer a flexible laser solution that can be equipped with 1 to 4 lasers. Laser lines can be upgraded at later stage as well.
As the RCM is sensitive, we only need low power lasers (max 25 mW). The advantage of the low power lasers is the significantly longer lifetime and lower costs.