NL5+
Mouse embryo in 140μm depth at E5.5 cultured in vitro for 24 hours. Stained with DAPI (blue), Phalloidin488 (green) and pHH3-647 (magenta).
Sample courtesy of Nanami Satoh, MRC LMB, Cambridge, UK
3D live cell imaging
with multiple filters
The NL5+ is the second generation of fast line-scanning confocal technologies. It provides high-contrast images from thicker specimens such as organoids, tissue samples, plant and animal model organisms. The gentle conditions for your live samples allow the NL5+ to excel in long time-lapse experiments and imaging low-signal samples.
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The combination of camera-based detection and a set of filters allows you to acquire your images in up to six different colors. This gives you an improved temporal resolution, high sensitivity and unprecedented signal-to-noise ratio. You get even faster imaging with cleaner results, thanks to additional motorized single-band emission filters.
Get better imaging
with the NL5+
- Integrated emission filter wheel (6 positions) for multiple colors
- See up to six different types of molecules per acquisition
- Get improved temporal resolution and higher sensitivity
- Get unprecedented signal-to-noise ratio
- Fully maintain the sectioning capability of your confocal microscope
- Get high-contrast images from thicker specimens
- Make excellent use of it by long time-lapse experiments
- Build a future-proof, budget-friendly confocal system for live cell imaging
Intestinal organoid, labeled with KI67 (blue), H2B-mCherry (green) and e-cadherin (red).
Sample courtesy of Pascal Ender from AMOLF, NL
The benefits of the
add-on filter
The NL5+ is a confocal system which enables even faster imaging with cleaner results. This is a direct result of one of the new functionalities: its additional motorized filter wheel.
Our system uses a line scanner, instead of a point scanner confocal microscope. This provides high temporal resolution, while at the same time reducing phototoxicity. Since phototoxicity is one of the biggest challenges for researchers, this makes the NL5+ the perfect tool for live cell imaging.
You can also add the NL5+ to any widefield microscope. This will turn it into an advanced fast-scanning confocal imaging system.
Discover how the NL5+ upgrades your imaging experience
The NL5+ applications
NL5+ has many perks that are similar to the NL5. For instance, the line scanning confocal system is easy to use. For individual research labs, you can easily convert widefield fluorescence microscopes into fast confocal microscope systems.
The NL5+ can capture datasets in 170nm lateral resolution using deconvolution on the fly. Minimal aberrations allow you to study molecular dynamics using speed up to 75 fps, or perform 3D imaging. The modern sCMOS cameras, which are used as detectors, feature high sensitivity up to 96%QE. They facilitate acquisition with low-light sample exposure.
The NL5+ in motion
This is the first impression of our latest confocal technology
NL5+: technical specs
NL5+ | |
|---|---|
Detector | Camera (sCMOS) |
Resolution in real time | 170 nm * |
Detector sensitivity | up to 96% QE |
FOV | FOV 18mm (FN18); 330×330 µm (40x objective) |
Speed in line scanning | up to 75 fps for 2048×512 px and up to 55 fps for 2048×2048 px** |
Speed in fixed line mode | 500 fps and more (camera limited) |
Wavelength | VIS (400-700 nm) |
Software | Micromanager, Inscoper, Volocity, NIS
Elements |
Deconvolution with | Microvolution (real time), SVI Huygens
(postprocessing) |
Modalities | Widefield and Confocal (motorized) |
Emission filters | 6 positions (field upgradable) |
Weight (kg) | 4.5kg |
Download your NL5+ application note
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