High contrast imaging. Low laser power.
In this movie healthy mitochondria and fast-acting dynamics can be observed after long-term image acquisition
Super-resolution imaging. Low laser power.
RCM1 is a highly sensitive super-resolution confocal system based on the Re-scan Confocal Microscope (RCM) principle. Capture datasets with 170nm raw resolution (120nm after deconvolution) by using 60x to 100x high numerical aperture (NA) magnification objectives and keep the laser intensity at a minimum. RCM1 acquires super-resolution images with only 85 nano-watts laser power.
Long-term mitochondria imaging with no signs of phototoxicity.
Higher sensitivity and less phototoxicity with RCM1
RCM1 enables you to investigate live-cell dynamics in high resolution while keeping the laser power extremely low. Its high sensitivity makes RCM exceptionally suitable for challenging biological applications where the signal is limited.
RCM1-NIR version facilitates deep tissue biomedical imaging, achieving much higher sensitivity in the near-infrared wavelengths than other systems. It works with 640nm and 785nm wavelengths which, due to the longer wavelength, are less phototoxic and penetrate much better into biological samples.
The benefits of using RCM1
Using low laser power increases cell survival and minimizes photobleaching during long-term time-lapses providing more relevant results. Unlike other super-resolution microscopy techniques, the RCM requires minimal light exposure for your samples!
Getting sharper and even higher contrast images, without averaging or integration, reduces the acquisition time and allows for a more precise analysis of the subcellular structures.
Hela cell stained for DNA (blue), Actin (green), Tubulin (red) and clathrin (yellow). Captured on RCM1 using a 100x 1.45 objective. Sample courtesy: Nicolas Touret, University of Alberta, Canada.
Who should use
RCM1 and why?
Obtain super-resolution confocal images with standard dyes and without complicated training. RCM1 is an easy-to-use and versatile system, great to add to any microscopy facility. It’s a fruitful solution for individual research labs to easily convert any existing widefield fluorescence microscope into a super-resolution and highly sensitive confocal system. Record living cells and organoids in 4D super-resolution using very low laser power. Don’t worry about phototoxicity or photobleaching. RCM1 provides optimized conditions for live-cell imaging and facilitates posterior analysis with sharp and high contrast raw images.
RCM1: technical specs
120 nm **
up to 95% QE
130×130 um (60x, super-resolution)
100x, 60x (high NA)
1fps @ 512 x 512 pixels
VIS or NIR
Micromanager, Nis Elements, Volocity
PSF for deconvolution with
Microvolution, SVI Huygens
** after deconvolution, raw image = 170nm
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