RCM2

Brighter images. Larger field of view.

RCM2
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In this movie mitochondria and actin dynamics are observed during long-term live cell imaging. RCM2 40x 1.4NA provided high contrast and a large field of view

Meet RCM2

Brighter images. Larger field of view.

RCM2 is our second generation RCM, with digital scanner technology. It makes bi-directional scanning the standard and allows a speed of 2fps at 512×512 pixels. RCM2 has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has demonstrated imaging at 10 nano-watt excitation power!

Improvements of sCMOS cameras allow you to sample resolution of low magnification objectives effectively, without increasing the exposure time. In a regular PMT-based confocal this is not possible.

Improve your imaging experience with RCM2

See how RCM2 improves your imaging experience for yourself

Confocal RCM2 Nikon Ti2 Side
Confocal Mitosis captured with RCM2
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HUVEC cells stained for SPY 505 DNA, SPY550 Actin and SPY650 tubulin.

Discover super-resolution imaging with RCM2

Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 4D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).

In combination with silicon objectives, RCM2 allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.

See how RCM2 improves your imaging experience for yourself

The benefits of RCM2

Using RCM2 with a 40x 1.4 objective, you can see more cells at full resolution at once. A larger field of view increases the chances of getting the results you need.

Obtain sharp images with a high signal-to-noise ratio even in samples with a low amount of epitopes or weak stainings. Get more from your samples.

Use even lower laser power to minimize phototoxicity and photobleaching during live-cell imaging.

Getting super-resolution raw images, without averaging or integration, reduces the acquisition time and allows for a more precise analysis of the subcellular structures.

See how RCM2 improves your imaging experience for yourself

RCM2 Neurons 40x

Neurons in co-culture stained for Actin (red), MAP2 (magenta) and Tau (green). Imaged on RCM2 with a 40x 1.4 objective. Sample courtesy: Vera Wiersma, VU University, Amsterdam, the Netherlands.

Who should use
RCM2 and why?

Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 4D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).

In combination with silicon objectives, RCM2 allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.

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RCM2: technical specs

RCM2
Detector
Camera
Resolution
120 nm **
Sensitivity
up to 95% QE
FOV
220×220 um (40x, super-resolution)
Optimized for
100x, 60x, 40x (high NA)
Scanner
Digital (closed-loop)
Speed
2fps @ 512×512 pixels
Wavelength
VIS
Software
Micromanager, Volocity, NIS Elements, Zen*, LAS X*, Cellsens
Integration
Hardware – USB connection
PSF for deconvolution with
Microvolution, SVI Huygens
Bypass mode
Yes

*still under investigation
** after deconvolution, raw image = 170nm

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