RCM2

Brighter images. Larger field of view.

rcm2
RCM2
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In this movie mitochondria and actin dynamics are observed during long-term live cell imaging. RCM2 40x 1.4NA provided high contrast and a large field of view

Brighter images.
Larger field of view

RCM2 is our second generation RCM, with digital scanner technology. It makes  re-scanning the standard and allows a speed of 2fps at 512×512 pixels. RCM2 has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has demonstrated imaging at 10 nano-watt excitation power!

Improvements of sCMOS cameras allow you to sample resolution of low magnification objectives effectively, without increasing the exposure time. In a regular PMT-based confocal this is not possible.

Improve your imaging experience with RCM2

See how RCM2 improves your imaging experience for yourself

Confocal RCM2 Nikon Ti2 Side
Confocal Mitosis captured with RCM2
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HUVEC cells stained for SPY 505 DNA, SPY550 Actin and SPY650 tubulin.

Discover super-resolution imaging with RCM2

Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 3D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).

In combination with silicon objectives, RCM2 allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.

See how RCM2 improves your imaging experience for yourself

The benefits of RCM2

Using RCM2 with a 40x 1.4 objective, you can see more cells at full resolution at once. A larger field of view increases the chances of getting the results you need.

Obtain sharp images with a high signal-to-noise ratio even in samples with a low amount of epitopes or weak stainings. Get more from your samples.

Use even lower laser power to minimize phototoxicity and photobleaching during live-cell imaging.

Getting super-resolution raw images, without averaging or integration, reduces the acquisition time and allows for a more precise analysis of the subcellular structures.

See how RCM2 improves your imaging experience for yourself

RCM2 Neurons 40x

Neurons in co-culture stained for Actin (red), MAP2 (magenta) and Tau (green). Imaged on RCM2 with a 40x 1.4 objective. Sample courtesy: Vera Wiersma, VU University, Amsterdam, the Netherlands.

Applications for RCM2

Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 3D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).

In combination with silicon objectives, RCM2 allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.

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RCM2: technical specs

RCM2
Detector
Camera (sCMOS)
Resolution in real time
120 nm *
Detector Sensitivity
up to 96% QE
FOV
FN12: 220×220 µm (40x objective), FN18 when not using super resolution
Speed
2 fps at 512 x 512 px, 15 fps in sprint level max at 256 x 256 px
Wavelength
standard VIS (400-700 nm), optional VIS-NIR (400-950 nm) with RCM2.5
Software
Micromanager, Inscoper, Volocity, NIS Elements, SDK available for integration on request
Deconvolution with
Microvolution (real time); SVI Hyugens (post processing)
Modalities
Super Resolution, Confocal, Widefield, Brightfield
Emission Filters
standard quad band, optional external single band filter wheel
Weight
23kg

*after deconvolution, raw image = 170nm

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