Call to all Microscopists: what laserpower are you using?
To kick off the “CHALLENGE” we would like to show a time lapse movie of HUVEC cells stained for GFP-mitochondria and SiR-DNA. As this is a real CHALLENGE we want to set the standards high. The laser power was measured to be 0.3 microwatt for the red channel and 0.085 microwatt for the green. This is 85 NANO watts! Optical resolution in the image is 120nm.
We challenge the microscopy community to show movies with even lower laser power. Please try to beat our 300 & 85 NANO watts. Please submit your entry here.