Imaging solutions

Discover the power of re-scan confocal microscopy

At Confocal.nl, we revolutionized imaging solutions with our top of the line microscopy technique: Re-scan Confocal Microscope (RCM). Super resolution, easy to use: meet RCM.

Revolutionary microscopy

Confocal.nl changed the way we look at things

Confocal microscopy changed the way we look at things
Confocal laser scanning microscopes were introduced in the seventies and literally changed the way we look at things. Confocal imaging increased the optical resolution and contrast by using a pinhole to block out-of-focus light, which sparked the concept of 3D imaging.

The confocal sectioning can capture multiple 2D images at different depths to reconstruct a three-dimensional structure within the same object or sample. This scientific breakthrough brought about a new and revolutionary approach to microscopy, which facilitated countless incredible discoveries.

Diffraction limit

Diffraction limit
However, the resolution was dependent on the diffraction limit. This means that two objects can only be distinguished if they are at a distance less than approximately half the wavelength of light used to image the specimen. Therefore, subcellular components like proteins or microorganisms like viruses or bacteria could not be resolved properly with this technology.

Advanced microscopy

Super-resolution microscopy
New microscopy technologies have been developed to break the diffraction limit and resolve smaller objects. This is called super-resolution microscopy. Some well known techniques are STORM, STED or SIM but they also present limitations, particularly when applied to live cell imaging experiments.

Confocal RCM

RCM goes beyond super-resolution

Other super-resolution techniques require very high laser power

A common disadvantage of the main super-resolution microscopy techniques is that the samples are exposed to high amounts of light. This is problematic for live cell imaging, as it generates phototoxicity, which can alter cell behaviour, morphology and lead to imprecise results. In addition, some techniques are dependent on special fluorophores and are difficult to execute for the average microscopy user, so an expert is often needed.

Confocal.nl goes one step further

At Confocal.nl, we took notice of these limitations, we listened to scientists’ needs and decided to go one step further. We took microscopy to the next level with our re-scanning confocal microscope (RCM). RCM goes beyond the diffraction limit, without generating phototoxicity and using normal fluorophores. Not to mention its user-friendliness and its straightforward character.

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