Fast & deep 3D live cell imaging

Meet NL5 video
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Volvox autofluorescence in 561 excitation. 100x 1.45 oil

Fast & deep 3D
live cell imaging

NL5 is a fast line-scanning confocal system with high sensitivity and resolution. It allows you to quickly screen a multi-well plate with multicolor images, from which you can select the most promising ones. Capture 3D live samples with minimal phototoxicity, high resolution and contrast. Like RCM, NL5 keeps your samples happy under the microscope for a long time and is easy to use. Convert your research microscope into a fast-scanning confocal microscope that provides very gentle conditions for your live samples.

How NL5 improves your imaging experience

Discover the way NL5 improves your imaging experience

NL5 benefit
Confocal fast scanning speed zebrafish
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Zebrafish embryo (24 to 48 hpf). Endothelial cells labelled with GFP Sample courtesy: Kinki Jim. VUmc Amsterdam, MMIC

Fast scanning speed, low levels of phototoxicity

In the field of biology, some natural processes happen fast, like cell and protein dynamics and cell-cell interactions. Therefore, fast scanning speed is needed to study them. And that is exactly what NL5 brings to the table! In addition, researchers in developmental biology, regeneration or immunology fields often need to image large live samples (mouse embryos, zebrafish, organoids) for a long time without losing any detail of the cell’s behavior. In that case, a combination of speed and a low phototoxicity system like NL5 is perfect.

Discover the way NL5 improves your imaging experience

The benefits of using NL5

NL5 is a fast scanning confocal system that reaches 55 fps (full frame), much faster than regular point-scanning confocal systems. In addition to speed, it brings very gentle imaging conditions for your samples as well. Perform live cell imaging for more than a day, with no signs of phototoxicity. NL5 is easy to use, and requires basic training to operate.

NL5 does not cause any pinhole cross-talk, as it has only one slit pinhole. Pinhole cross-talk is associated with spinning disk systems, because they contain a disk with multiple pinholes combined and the out-of-focus light can sometimes pass through the closeby pinholes. This effect is more evident when imaging deep within the sample, making NL5 the ideal system for those applications.

NL5 is a cost-effective solution that can be coupled to any research microscope via the c-mount interface

HeLa cell
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HeLa Cell with cytosolic GFP. Sample courtesy of Caroline Pham (Lodder’s Group). Experimental Cardiology Department. VUmc location AMC, Amsterdam

Applications for NL5

High resolution & low phototoxicity

NL5 is an easy-to-use and compact confocal microscope system, a great addition to any microscopy facility. It’s a fruitful solution for individual research labs to easily convert any existing widefield fluorescence microscope into a fast confocal microscope system with high sensitivity and resolution. Capture datasets of 170nm lateral resolution after deconvolution at 55 fps. Study fast cell and molecular dynamics, or perform high contrast deep 3D imaging with minimal aberrations. The high sensitivity of the modern sCMOS cameras used as detectors facilitates acquisition with low light sample exposure, making NL5 ideal for live cell imaging.

Discover the way NL5 improves your imaging experience

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NL5: technical specs

Resolution in real time
170 nm *
Detector sensitivity
up to 96% QE
FOV 18mm (FN18); 330×330 µm (40x objective)
Speed in line scanning
up to 75 fps for 2048×512 px and up to 55 fps for 2048×2048 px**
Speed in fixed line mode
500 fps and more (camera limited)
VIS (400-700 nm)
Micromanager, Inscoper, Volocity, NIS Elements
Deconvolution with
Microvolution (real time), SVI Huygens (postprocessing)
Widefield and Confocal (motorized)
Emission filter
1 position (standard with Quadband)
* using deconvolution
** measured with Kinetix camera (Teledyne) on a biological sample
Confocal demo form image

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