- Confocal imaging – RCM has improved lateral resolution and the same axial resolution compared to conventional confocal microscope.
- Live cell imaging – RCM has a very high signal-to-noise ratio and quantum efficiency (80-95%, depending on the camera), which means no need for high laser power.
- Ratio-imaging and multi-color applications – RCM can work in multi-colour mode for different colour combinations and ratio-imaging (applications like FRET, FRAP, pH, and Ca2+ imaging).