Deconvolution Representative nuclear spread from fixed mouse spermatocytes, immunostained for SYCP3 a component of the synaptonemal complex (Alexa 488-labelling) imaged with RCM using without re-scanning (confocal) and with re-scanning (RCM). Left: full spread. Right: individual chromosomes. Sample courtesy of A. Agostinho – Advanced Light Microscopy Facility, Science for Life LaboratoryC. Höög – Department of Cell and Molecular Biology, Karolinska Institutet. Imaging and deconvolution by Erik Manders (Confocal.nl). For deconvolution SVI Huygens software was used. N1E-115 neuroblastoma cells, made during HOLM 2017 by the students attending one of the workshops. On the left, raw image from RCM, on the right the same image after the Microvolution deconvolution. Mitotic cell – Cos7 cell line with antibody labeling of nucleus (blue), mitochondria (green), and tubulin (red). Samples prepared by Jana Doehner (University of Zurich). Images taken by Stan Hilt (Confocal.nl) during the BioImaging meeting Utrecht. Original RCM image (left), and the result of NIS Elements deconvolution (right).